Specific Inhibition of Coxsackievirus B3 Translation and Replication by Phosphorothioate Antisense Oligodeoxynucleotides

Specific inhibition of coxsackievirus B3 translation and replication by phosphorothioate antisense oligodeoxynucleotides.

The 5' and 3' untranslated regions (UTRs) of coxsackievirus B3 (CVB3) RNA form highly ordered secondary structures that have been confirmed to play important regulatory roles in viral cap-independent internal translation initiation and RNA replication. We previously demonstrated that deletions in different regions of the 5' UTR significantly reduced viral RNA translation and infectivity. Such o...

Effect of Activation and Inhibition of Cellular PKR on Coxsackievirus B3 Replication

The ds-RNA activated protein kinase (PKR) is a serine-threonine kinase with MW of 68 KDa. It belongs to a family of kinases that control one of the translational initiation factors, eIF2. PKR is produced at high level in response to viral infection. This protein by phosphorylating eIF2 inhibits cellular protein synthesis. In this study, the effect of gamma interferon (IFN-γ), an activator, and ...

Effect of Host Src-kinase Inhibition on Replication of Coxsackievirus B3 in vitro

Translation of stable hepadnaviral mRNA cleavage fragments induced by the action of phosphorothioate-modified antisense oligodeoxynucleotides

Phosphorothioate-modified antisense oligodeoxynucleotides (ASOs) are used to suppress gene expression by inducing RNase H-mediated cleavage with subsequent degradation of the target mRNA. However, previous observations suggest that ASO/RNase H can also result in the generation of stable mRNA cleavage fragments and expression of truncated proteins. Here, we addressed the underlying translational...

effect of activation and inhibition of cellular pkr on coxsackievirus b3 replication

the ds-rna activated protein kinase (pkr) is a serine-threonine kinase with mw of 68 kda. it belongs to a family of kinases that control one of the translational initiation factors, eif2. pkr is produced at high level in response to viral infection. this protein by phosphorylating eif2 inhibits cellular protein synthesis. in this study, the effect of gamma interferon (ifn-γ), an activator, and ...